Lactobacillus crispatus UMB6490 - SRA

SRX25216360: Lactobacillus crispatus UMB6490
1 ILLUMINA (NextSeq 2000) run: 905,073 spots, 268.2M bases, 93.8Mb downloads

Design: DNA was extracted using the Qiagen DNeasy Blood and Tissue Kit, following the manufacturers protocol for Gram-postive species. Samples were prepared for whole genome sequencing using the Illumina DNA Prep tagmentation kit and unique dual indexes. Sequencing was performed on the Illumina NextSeq2000 platform using a 300 cycle flow cell kit to produce 2x150bp paired reads. 1-2% PhiX control was spiked into the run to support optimal base calling. Read demultiplexing, read trimming, and run analytics were performed using DRAGEN v3.10.12.

Submitted by: Loyola University Chicago

Study: Female Urinary Microbiota Genome Sequencing

PRJNA316969 • SRP072679 • All experiments • All runs

show Abstracthide Abstract

Recently, bacterial communities (microbiota) have been discovered in the female bladder. Thus, the “sterile urine” paradigm is no longer valid. In an effort to provide a comprehensive view of the newly discovered female urinary microbiota (FUM), we have established an Enhanced Quantitative Urine Culture (EQUC) protocol. EQUC isolates bacteria from 75-90% of urine samples deemed 'no growth' by the standard clinical microbiology urine culture method. We have begun to sequence and annotate the genomes of these isolated bacteria.

Sample:

SAMN42334631 • SRS21903638 • All experiments • All runs

Organism: Lactobacillus crispatus

Library:

Name: Lc_UMB6490

Instrument: NextSeq 2000

Strategy: WGS

Source: GENOMIC

Selection: RANDOM

Layout: PAIRED

Runs: 1 run, 905,073 spots, 268.2M bases, 93.8Mb

Run	# of Spots	# of Bases	Size	Published
SRR29713983	905,073	268.2M	93.8Mb	2024-07-05

ID:: 33557272

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